Quick Summary
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A DNA profile of species-specific microsatellite markers is obtained from the offspring, potential dam, and potential sire(s), and parentage analysis is performed using this data. In this process, a computer program compares the DNA profile of the offspring to the profiles of the presumed parents. Candidate sires and dams may either qualify or be excluded as parents based on whether they share microsatellite marker alleles with the offspring. A parentage analyst then reviews the results and sends the final report. If a listed parent or parents are excluded, additional analysis is performed, including retesting of samples and the possible use of additional DNA markers to confirm the exclusion.
You can read more about the process of DNA parentage analysis HERE.
Sample Collection:
Cat DNA tests are carried out using cells collected from your cat's cheeks and gums using household cotton swabs.
The cat DNA submission form with instructions, pricing, and a place to tape the cotton swabs can be printed from your home computer after signing up for a MyVGL account and placing an order. Test kits are not mailed.
Step-By-Step Instructions:
1.
Purchase regular household cotton swabs for cat DNA collection (the cotton swabs can be purchased at a pharmacy or drug store)
2.
Use both ends of the two cotton swabs for a total of four swabs.
3.
Collect the DNA sample by swabbing the cheek and gums of the cat.
4.
After swabbing the cheek and gums, tape the cotton swabs to the bar-coded submission form printed from your MyVGL account.
In cats, the following microsatellite markers are reported:
FCA005
FCA224
FCA229
FCA293
FCA310
FCA441
FCA453
FCA649
FCA678
FCA026
FCA069
FCA075
FCA097
FCA105
FCA149
FCA201
FCA220